| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carvajal, Kailey H (14)
|
VACGBD |
55.51 |
57.45 |
1.94 |
| 2 |
Rose, Mia G (14)
|
NCNCAC |
56.09 |
59.66 |
3.57 |
| 3 |
Eward, Aelwyn C (13)
|
NCNCAC |
58.61 |
1:00.25 |
1.64 |
| 4 |
Kimbel, Isabella M (13)
|
VACGBD |
55.93 |
1:00.82 |
4.89 |
| 5 |
Gaffney, Laura M (13)
|
VACGBD |
1:00.00 |
1:00.92 |
0.92 |
| 6 |
Febre, Kirsten M (14)
|
NCUN |
1:00.63 |
1:01.13 |
0.50 |
| 7 |
Nguyen, Jackqueline T (13)
|
NCNCAC |
1:00.31 |
1:01.61 |
1.30 |
| 8 |
Denny, Britney L (13)
|
VACGBD |
59.96 |
1:01.77 |
1.81 |
| 9 |
Mangum, Sarah M (14)
|
VACGBD |
1:00.73 |
1:02.40 |
1.67 |
| 10 |
Gere, Sophie I (14)
|
VACGBD |
1:00.48 |
1:03.44 |
2.96 |
| 11 |
Trusky, Catherine C (14)
|
NCNCAC |
1:00.73 |
1:03.45 |
2.72 |
| 12 |
Bilden, Lindy C (13)
|
NCNCAC |
1:04.22 |
1:04.37 |
0.15 |
| 13 |
Coppage, Ashley G (14)
|
VACGBD |
1:04.69 |
1:04.47 |
-0.22 |
| 14 |
Younger, Delaney L (13)
|
VACGBD |
1:04.71 |
1:04.58 |
-0.13 |
| 15 |
Watson, Samantha L (14)
|
VACGBD |
1:02.60 |
1:05.39 |
2.79 |
| 16 |
Neikirk, Alexandra L (14)
|
VACGBD |
1:03.99 |
1:06.02 |
2.03 |
| 17 |
Guerry, Hayley A (13)
|
NCNCAC |
1:06.45 |
1:09.43 |
2.98 |
|
Aubrey, Sofia S (13)
|
NCNCAC |
59.12 |
NS |
- |
|
Liu, Rachel S (13)
|
NCNCAC |
1:19.95 |
NS |
- |
|
O'Brien, Anne E (14)
|
NCNCAC |
1:06.72 |
NS |
- |
|
Qin, Annie L (13)
|
NCNCAC |
1:03.97 |
NS |
- |
|
Wright, Abigail N (13)
|
NCNCAC |
1:26.17 |
NS |
- |